New Products
Total RNA and DNA from FFPE samples
		NucleoSpin® FFPE RNA/DNA – Paralell isolation of RNA and DNA from FFPE samples Features: • RNA and DNA from one unsplit FFPE sample – direct correlation – no deviations • Very easy deparaffinization method – no xylene needed • Paraffin Dissolver (patent pending) and decrosslinking buffer included • Improved RNA and DNA quality and concentration – enhanced performance in all    common downsteam applications • Compatible with archived clinical samples NucleoSpin® FFPE RNA/DNA allows the parallel isolation of both, RNA and DNA from the same specimen without sample splitting. DNA and RNA are eluted sequentially and selectively into two separate fractions. The kit introduces an innovative method for deparaffinization using the Paraffin Dissolver (patent pending). Formaldehyde modifications (crosslinks) are removed efficiently by incubation with a decrosslinking buffer. Concentrated RNA can be eluted in as little as 5 µl and DNA is eluted in 20 µl. RNA and DNA isolated with NucleoSpin® FFPE RNA/DNA are ready to use in all common downstream applications leading to excellent performances in RT-PCR or PCR. Product page NucleoSpin FFPE® RNA/DNA
Genomic DNA from Soil
		NucleoSpin® Soil - Kit for total DNA isolation from soil, sediment, or sludge NucleoSpin® Soil is a kit for isolation of total DNA from different types of soil samples. The kit includes four different lysis systems which make the kit suitable for a broad range of different samples. This is important as soil types are very heterogenous and contain a lot of different organic or anorganic substances like humic substances, polysaccharides, or acidic components. NucleoSpin® Soil allows processing of up to 500 mg (wet weight) starting material. The eluted DNA is ready to use (undiluted) for subsequent reactions like PCR, restriction analysis, and others. Features: • Alternative lysis systems    Two different lysis buffers and an optional additive allow to tune for the best result • Most efficient cell disruption    Ready-to-use NucleoSpin Bead Tubes with ceramic beads included • Complete removal of PCR inhibitors    NucleoSpin Inhibitor Removal Columns and efficient wash buffers eliminate PCR inhibitors    DNA can mostly be used undiluted as PCR template Product page NucleoSpin® Soil
Purification of GST-tagged proteins
		Protino® GST/4B Columns 5 ml - Save money without making compromises The new product of the Protino® line for purification of GST-tagged proteins features proven market-leading performance for an unbeatable price. With Protino® GST/4B Columns 5 ml MACHEREY-NAGEL offers a scale up of the Protino® GST/4B Columns 1 ml as the cost-effective alternative for MPLC-/FPLC™-based purification of GST-tagged proteins. The new Protino® GST/4B Columns 5 ml are filled with Protino® Glutathione Agarose 4B matrix and allow consistent upscaling from 1 ml to 5 ml without accepting any compromise. Protino® GST/4B Columns 5 ml show the same technical specifications and performance as the market leading product GSTrap™ 4B 5 ml columns from GE Healthcare - but, as well as the resin, with great savings in price. Product page Protino® GST/4B Columns 5 ml
Genomic DNA from Blood
		NucleoSpin® Dx Blood – IVD-CE marked kit for genomic DNA isolation from blood NucleoSpin® Dx Blood is a mini spin kit for the isolation of genomic DNA from 200 µl whole blood. The kit is CE-IVD marked in accordance to the directive 98/79/EC of the European Union, guaranteeing highest standards of production and quality assurance. NucleoSpin® Dx Blood fits into in-vitro diagnostic workflows*. It is suitable for fresh and frozen blood samples and compatible with common blood collection systems and anticoagulants, including EDTA, citrate, and heparin. Features: • CE-marked in accordance with EU Directive 98/79/EC    Compliance with IVD directives in the EU • Fits into in-vitro diagnostic workflows    Can be combined with any enzymatic amplification and detection of DNA • Validated procedure for DNA isolation from whole blood    Reproducible results for reliable downstream applications • Compatible with common blood collection tubes and anticoagulants    Suitable for EDTA, citrate, and heparin blood collection systems    For fresh and frozen blood samples • Convenient handling    Product storage: at room temperature    Azide-free elution buffer: Easy and reliable photometric DNA quantification *CE-IVD marked kit in compliance with EU directive98/79/EC. Only available as IVD in certain countries.    All other countries research use only! Please inquire.  Product page NucleoSpin® Dx Blood
Total RNA and/or microRNA isolation
		NucleoSpin® miRNA – Parallel isolation of small RNA, large RNA, and protein NucleoSpin® miRNA is suitable for simultaneous isolation of small RNA (< 200 nt), large RNA (> 200 nt), and protein in three separate fractions from a large variety of sample materials. The kit offers the unique feature to isolate separate fractions of small and large RNA as well as proteins without the need of cumbersome phenol/chloroform extraction. Gene expression can thus be analyzed on the level of transcription (mRNA or miRNA) and on the level of translated proteins to get most reliable results. Superior selectivity - purify your RNA fractionated by size...highest flexibility! Additional isolation of total protein...confirm RNA results on protein level! Excellent recovery - even without phenol/chloroform...less hazardous! Convenient handling - complete equipment provided...ease of use! Product page NucleoSpin® miRNA
Total RNA from FFPE samples
		NucleoSpin® FFPE RNA – Get RNA easily out of FFPE samples Features: • Very easy deparaffinization method • Paraffin Dissolver (patent pending) and decrosslinking buffer included • Minimum number of working steps – fast and easy handling • Improved RNA quality and concentration – enhanced performance in RT-PCR • Compatible with archived clinical samples The NucleoSpin® FFPE RNA kit has been developed to ease and improve RNA isolation from FFPE samples. It introduces an innovative method for deparaffinization using the Paraffin Dissolver (patent pending). Formaldehyde modifications (crosslinks) are removed efficiently by incubation with a decrosslinking buffer and concentrated RNA of improved quality can be eluted in as little as 5 – 30 µl. RNA, isolated with NucleoSpin® FFPE RNA is ready to use in downstream applications and leads to excellent RT-PCR performances. Product page NucleoSpin® FFPE RNA
Isolation of RNA, DNA, and protein
		NucleoSpin® TriPrep - Parallel isolation of RNA, DNA, and protein from unsplit samples NucleoSpin® TriPrep allows the parallel isolation of RNA, DNA, and protein from a single, unsplit sample and gives three results from one experiment: A) DNA: Highly pure DNA can be isolated, ready to use in downstream applications.  B) RNA: The isolated RNA is excellent in integrity, purity, and yield. For best performance      and convenience rDNase for on-column DNA digest is provided with the kit  C) Protein: The isolated protein is ready to use for SDS-PAGE and Western blotting.      Poteins are preserved in quality and phosphorylation. NucleoSpin® TriPrep is suitable for a broad spectrum of starting materials, including cultured cells, tissue, bacteria, yeast, and plants. The rapid isolation of RNA, DNA, and protein from one and the same sample without sample splitting makes this kit ideal for unique, small, and precious samples. Product page NucleoSpin® TriPrep KITS TO BE USED FOR RESEARCH PURPOSES ONLY. DISTRIBUTION AND USE IN THE USA IS PROHIBITED FOR PATENT REASONS.
Protein quantification
		Protein Quantification Assay - For protein quantification at high detergent and reducing agent concentrations The Protein Quantification Assay is a convenient and reliable kit for the determination of protein concentration in buffers typically used for SDS-PAGE (sodium dodecylsulfate polyacrylamide gel electrophoresis). It is mainly designed for proteins solved in Protein Solving Buffer (PSB), a component of NucleoSpin® RNA/Protein, and is suitable for proteins solved in buffer as described by Laemmli (1970), or similar. Protein quantification can be carried out in microplates, microcuvettes, semi-microcuvettes, and with low-volume photometers (e.g., NanoDrop™) within only 40 minutes. Product page Protein Quantification Assay
Purification of GST-tagged proteins
		Protino® GST/4B Columns 1 ml and 5 ml- Save money without making compromises The new product of the Protino® line for purification of GST-tagged proteins features proven market-leading performance for an unbeatable price. With Protino® GST/4B Columns 1 ml MACHEREY-NAGEL offers the cost-effective alternative for MPLC-/FPLC™-based purification of GST-tagged proteins. The new Protino® GST/4B Columns 1 ml are filled with Protino Glutathione Agarose 4B matrix and show the same technical specifications and performance as the market leading product GSTrap™ 4B 1 ml columns from GE Healthcare - but, as well as the resin, with great savings in price. Product page Protino® GST/4B Columns 1 ml