NucleoBond BAC 100 kit for large construct plasmid DNA, MN

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Delivery time approx. 5 working days

Item number: 740579 031010410001

Package unit 10 Preps

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1st generation anion exchange technology for large construct plasmid DNA more

1st generation anion exchange technology for large construct plasmid DNA

Application	Large contruct (P1, BACs, PACs) plasmid purification
Target	Plasmid DNA
CE certified	No, research use only
Technology	Anion exchange chromatography
Brand	NucleoBond
Format	BAC prep
Handling	Gravity flow
Lysate clarification	Folded filter
Automated use	No
Sample material	Bacteria, E. coli
Sample amount	100−500 mL E. coli culture
Vector size	< 300 kbp
Typical yield	10−100 µg
Theoretical binding capacity	100 µg
Typical purity A260/A280	1.8−1.95
Endotoxin level	1−10 EU/µg DNA
Isopropanol precipitation	Centrifugation
Preparation time	120 min/2−4 preps
Typical downstream application	Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature	15–25 °C / 59–77 °F
Shelf life (from production)	39 Month(s)
Scope of delivery	NucleoBond BAC 100 Columns, NucleoBond Folded Filters, buffers, RNase A
Gross weight (incl. packaging)	2326.3 g / 5.13 lbs
Packaging dimensions	215 x 215 x 165 mm / 8.46 x 8.46 x 6.50 Inch
Hazardous material	Yes