NucleoBond PC 100, Midi kit for transfection-grade plasmid DNA, MN

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Item number: 740573.100 031010410001

Package unit 100 Preps

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1st generation anion exchanger for purification of plasmid DNA more

1st generation anion exchanger for purification of plasmid DNA

Application	High and low copy plasmid DNA purification
Selling unit	20 Prep(s), 100 Prep(s)
Target	Plasmid DNA
CE certified	No, research use only
Technology	Anion exchange chromatography
Brand	NucleoBond
Format	Midi prep
Handling	Gravity flow
Lysate clarification	Folded filter
Automated use	No
Sample material	Bacteria, E. coli
Sample amount	5−30 mL (high copy), 10−100 mL (low copy)
Vector size	< 300 kbp
Typical yield	20−100 µg
Theoretical binding capacity	100 µg
Typical purity A260/A280	1.8−1.95
Endotoxin level	1−10 EU/µg DNA
Isopropanol precipitation	Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi
Preparation time	65 min/4−6 preps
Typical downstream application	Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature	15–25 °C / 59–77 °F
Shelf life (from production)	39 Month(s)
Hazardous material	Yes