NucleoMag Plasmid, kit for plasmid DNA isolation with magnetic beads
*taxes and shipping not included
The NucleoMag Plasmid Kit is ideal for automated isolation of plasmid DNA on magnetic rod systems such as MagnetaPure 32 Plus or IsoPure Mini. Typical downstream applications with the isolated plasmid DNA are sequencing, cloning, PCR, transformation, transfection, in vitro transcription, ligation.
| Fragment size | < 25 kbp |
| Typical purity A260/A230 | ≥ 2.0 |
| Application | High copy plasmid DNA purification |
| Selling unit | 96 Preps, 384 Preps |
| Target | Plasmid DNA |
| CE certified | No, research use only |
| Technology | Magnetic bead technology |
| Brand | NucleoMag |
| Format | Magnetic beads |
| Handling | Automated |
| Lysate clarification | Centrifugation, Magnetic Beads |
| Automated use | Yes |
| Sample material | Bacteria, E. coli |
| Sample amount | ≤ 5 mL E.coli culture |
| Typical purity A260/A280 | ≥ 1.8 |
| Endotoxin level | ≤ 50 EU/µg DNA - Lysate clearance via magnetic beads, ≤ 50 EU/µg DNA - Lysate clearance via centrifugation (3 wash steps), ≤ 10 EU/µg DNA - Lysate clearance via centrifugation (4 wash steps) |
| Elution volume | 50–200 µL |
| Typical downstream application | Cloning, In-vitro transcription, PCR, Restriction analysis, Sequencing, Transfection of cells, Transformation |
| Shelf life (from production) | 15 Month(s) |
| Hazardous material | Yes |
NucleoMag Plasmid
Automated plasmid purification with transfection-grade purity
Plasmid DNA serves as an important tool in genetic research, allowing scientists to manipulate genes for various purposes, understand biological processes, and develop novel therapies. However, the journey to obtain high-quality plasmid DNA can be challenging due to potential contamination and degradation that can compromise the integrity of the genetic material. The NucleoMag Plasmid kit presents an effective solution by simplifying the plasmid isolation process. Utilizing innovative magnetic rod systems, this kit streamlines plasmid DNA extraction while maintaining the purity and efficiency necessary for robust scientific investigations. By automating the isolation process, researchers can achieve consistent results without the need for constant monitoring. This not only saves time but also allows scientists to focus on other essential tasks at the same time.
IsoPure Mini*
- Compact footprint
- Automation benefits
- Android app compatible
- Process 16 samples in parallel
* only available in the US
MagnetaPure 32 Plus
- User-friendly with saved methods
- Automation benefits
- Open platform for flexibility
- Process 32 samples in parallel
Application data – Isolation of transfection-grade plasmid DNA
High yields and consistency with automation
Plasmid DNA from 2 mL bacterial cells (E.coli) cultivated in LB medium was isolated using the NucleoMag Plasmid kit. The kit was processed on the MagnetaPure 32 Plus, a magnetic rod system for automated nucleic acid extraction (n=8). Three different vectors were isolated, including pCMV-GFP, pcDNA3.1 and pGEM T-Easy, resulting in high and consistent plasmid DNA yields. Lysate clarification was performed via centrifugation.
Ideal puritiy ratios for your downstream application
Plasmid DNA from 2 mL bacterial cells (E.coli) cultivated in LB medium was isolated using the NucleoMag® Plasmid kit. The kit was processed on the MagnetaPure 32 Plus, a magnetic rod system for automated DNA/RNA extraction (n= 8). Figure A shows A260/A280 purity ratios of high consistency across different vectors and preparations. Figure B displays the purity ratio A260/A230. Results show A260/A230 ratios are consistently ≥ 2.0 for all isolated plasmids. Lysate clarification was performed via centrifugation.
Technical data & contact information
Please follow the link below or read the product brochure for more information on technical data, specifications and related products.
NucleoMag Plasmid product features at a glance
- Automated plasmid isolation with magnetic rod systems
- Highest consistency and reliability
- Plasmid DNA up to 50 µg
- Flexible: Two options for lysate clarification based on your needs
- Suitable for transfection, sequencing, cloning, etc.
Speak to an expert
Questions about MN’s reagents for automation, scripting support or automation service? Please contact us for personal assistance!
Phone: +49 2421 969 333
Application data – Comparison with other magnetic bead based plasmid isolation kits
High yields and purities
Plasmid DNA from 1.5 mL bacterial cells (E.coli) cultivated in LB medium was isolated using NucleoMag Plasmid and a magnetic bead based kit from Supplier O. Plasmid isolation was conducted on the IsoPure Mini (n= 4). A pCMV-GFP vector was isolated using the 4 wash step protocol. Lysate clarification was performed via centrifugation for both kits. The figure shows total DNA yields in µg as well as A260/A280 and A260/A230 purity ratios.
NucleoMag Clearing Beads as second processing option
Plasmid DNA from 750 µL bacterial cells (E.coli) cultivated in LB medium was isolated using NucleoMag Plasmid and a magnetic bead based kit from Supplier Z. Plasmid isolation was conducted on IsoPure Mini (n= 4). A pCMV-GFP vector was isolated following each manufacturer’s recommended protocols. Lysate clarification was performed via magnetic beads for both kits. The figure shows total DNA yields in µg as well as A260/A280 and A260/A230 purity ratios.
| Product | EU/µg DNA |
|---|---|
| NucleoMag Plasmid (Clarification via centrifugation) | 4.3 ± 1.7 |
| NucleoMag Plasmid (Clarification via beads) | 10.9 ± 1.3 |
| Supplier O (Clarification via centrifugation) | 18.4 ± 19.7 |
| Supplier O (Clarification via beads) | 18.9 ± 9.8 |
| Supplier Z (Clarificationvia beads) | 3757 ± 418 |
Low endotoxin levels with NucleoMag Plasmid for both processing options
Plasmid DNA was isolated according to each manufacturer’s recommended protocols. Endotoxin units (EU) were determined by a quantitative chromogenic LAL-test (n=4).
Ordering information
| Product | Pack of | REF |
|---|---|---|
| NucleoMag Plasmid | 96 preps / 384 preps | 744750.1 |
| NucleoMag Clearing Beads | 2 × 1.75 mL (96 preps) | 744751.1 |
| 96 Deep-well plates for magnetic rod systems | 25 | 744955 |
| 8-well Tip Combs for magnetic rod systems | 50 | 744960 |
| Culture Plate (with Gas-permeable Foil) | 4 sets / 24 sets | 740488 / 740488.24 |
Comparison tests: NucleoMag Plasmid vs. Competitor Kit (T)
Plasmid DNA from 1.5 mL bacterial cells (E.coli) cultivated in LB medium was isolated using NucleoMag Plasmid and a magnetic bead based kit from Supplier T. A pcDNA3.1 vector was isolated. The figure shows total DNA yields in μg as well as A260/A280 and A260/A230 purity ratios. Lysate clarification was performed via centrifugation for both kits (dark blue and orange bars). Nucleo-Mag Plasmid CB demonstrates the performance of the Nucleo-Mag Plasmid kit using NucleoMag Clearing Beads (light blue bars).
Plasmid DNA from 1.5 mL bacterial cells (E.coli) cultivated in LB medium was isolated using NucleoMag Plasmid and a magnetic bead based kit from Supplier T. A pCMV-GFP vector was isolated. The figure shows total DNA yields in μg as well as A260/A280 and A260/A230 purity ratios. Lysate clarification was performed via centrifugation for both kits (dark blue and orange bars). Nucleo-Mag Plasmid CB demonstrates the performance of the Nucleo-Mag Plasmid kit using NucleoMag Clearing Beads (light blue bars).
Endotoxin levels
| Product | EU/μg DNA (pcDNA 3.1 (+)) | EU/μg DNA (pCMV-GFP) |
|---|---|---|
| NucleoMag Plasmid (Clarification via centrifugation) | 0.318 ± 0.2 | 4.195 ± 1.1 |
| NucleoMag Plasmid (Clarification via beads) | 4.805 ± 1.4 | 17.815 ± 1.2.7 |
| Supplier T (Clarification via centrifugation) | 1063 ± 4.3 | 1228 ± 6.8 |
Plasmid DNA was isolated according to each manufacturer´s recommended protocols. Endotoxin units (EU) were determined by a quantitative chromogenic LAL-test (n=4).
