NucleoSpin Blood, Mini kit for DNA from blood
*taxes and shipping not included
Mini spin kit for versatile purification of high quality DNA from blood
Application | Isolation of DNA |
Selling unit | 10 Prep(s), 50 Prep(s), 250 Prep(s) |
Target | DNA |
CE certified | No, research use only |
Technology | Silica membrane technology |
Brand | NucleoSpin |
Format | Mini prep |
Handling | Centrifugation |
Automated use | No |
Sample material | Blood, Body fluids, Buffy coat, Cells, Plasma, Platelets, Serum |
Sample amount | 5–200 µL whole blood (human or animal, fresh or frozen, treated with citrate, EDTA, heparin, or CPDA), buffy coat, platelets, body fluids, serum, plasma, < 5 x 10⁶ human/animal cells cultured cells |
Fragment size | 200 bp–approx. 50 kbp |
Typical yield | 4–6 µg |
Typical concentration | 40–100 ng/µL |
Theoretical binding capacity | 60 µg |
Typical purity A260/A280 | 1.6–1.9 |
Elution volume | 60–200 µL |
Preparation time | 30 min/prep |
Typical downstream application | enzymatic reactions, Next Generation Sequencing, PCR, Southern blotting |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 27 Month(s) |
Hazardous material | Yes |
NucleoSpin Blood
- Ready-to-use DNA in less than 30 min
- Complete removal of PCR inhibitors
- Suitable for whole blood, treated with citrate, EDTA, heparin, CPDA
- Consistently high DNA yields
NucleoSpin Blood - Procedure
NucleoSpin Blood kits are designed for rapid purification of genomic DNA from whole blood, serum, plasma, and body fluids (amniotic fluid, liquor etc.). Animal or human blood samples, fresh or frozen material, can be processed conveniently.
The NucleoSpin Blood method is based on a specially treated silica membrane with a high binding capacity for nucleic acids. The obtained DNA can be used directly for PCR (e.g., HLA typing), Southern blotting, or any kind of enzymatic reactions.
The kit allows the isolation of 4–6 μg of pure genomic DNA from 200 μL whole blood with an A260/A280 ratio between 1.60 and 1.90 and a typical concentration of 40–60 ng per μL. Due to the high binding capacity of the NucleoSpin Blood membrane, yields of up to 60 μg DNA are possible (from starting materials like buffy coat or lymphocyte-enriched samples).
With the NucleoSpin Blood method, genomic DNA is prepared from whole blood. Lysis is achieved by incubation of whole blood in a solution containing large amounts of chaotropic ions in the presence of proteinase K at 70 °C. Appropriate conditions for binding of DNA to the silica membrane of the NucleoSpin Blood columns are created by addition of ethanol to the lysate.
Superior purification with the NucleoSpin Blood kit
DNA was isolated from human blood samples (n = 3) using the NucleoSpin Blood kit and competitor kits from T, AJ and Q (light grey bars for A₂₆₀/A280 and dark grey bars for A260/A230 ratios). The purity was determined by UV-spectrometry resulting in an average A260/A280 value for the NucleoSpin Blood kit of 1.89 ± 0.03 (dark blue bar) and an average A260/A230 value (light blue bar) of 1.88 ± 0.03.
Competitive sensitivity measured by qPCR
DNA was extracted from human blood samples the with NucleoSpin Blood kit (dark blue bar) and the competitor kits from T, AJ and Q (grey bars). Samples were analyzed in triplicate by qPCR for β-globin (268 bp). With an average amplification cycle of 23.1 the results demonstrate the competitive performance and reliably high quality of DNA extraction with the NucleoSpin Blood kit.