NucleoSpin RNA Stool, Mini kit for RNA from stool
*taxes and shipping not included
Fast isolation of total RNA from various stool specimen, MN Bead Tubes Type A included
Application | Isolation of total RNA |
Selling unit | 10 Prep(s), 50 Prep(s) |
Target | RNA |
CE certified | No, research use only |
Technology | Silica membrane technology |
Brand | NucleoSpin |
Format | Mini prep |
Handling | Centrifugation, vacuum |
Lysate clarification | Centrifugation |
Automated use | No |
Sample material | Stool |
Sample amount | 180−220 mg |
Fragment size | > 18 nt |
Typical yield | 10–30 µg |
Theoretical binding capacity | 200 µg |
Typical purity A260/A280 | 1.9–2.1 |
Typical purity A260/A230 | 1.8−2.5 |
Typical RIN | > 7.5 |
Elution volume | 100 µL |
Preparation time | 70 min/10 preps |
Typical downstream application | Array technology, Northern blotting, Primer extension, Real−time RT−PCR, RNase protection assays |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 24 Month(s) |
Hazardous material | Yes |
- Download Instruction NucleoSpin RNA Stool
- Download Protocol recommendations for viral nucleic acid isolation from water samples
- Download Protocol recommendation sample concentration for viral nucleic acids from water
- Download Flyer NucleoSpin RNA Stool
- Download Flyer NucleoSpin RNA Stool (ES)
- Download Leaflet
RNA purification from stool samples - NucleoSpin RNA Stool
A combination of mechanical homogenization and chemical lysis ensures optimized RNA yield, including small RNA. NucleoSpin Inhibitor Removal Columns efficiently remove PCR inhibitors.
NucleoSpin RNA procedure
Superior RNA yield and qPCR performance
Highest yield with NucleoSpin RNA Stool
Two human stool samples (250 mg; MN only 200 mg) were processed in triplicates following the standard protocols including the DNase digestion step. The Q protocol was performed including the optional phenol based lysis step. Each eluate was used for UV spectroscopy to determine RNA yield. The NucleoSpin RNA Stool showed the highest RNA yield for the human stool samples.
NucleoSpin RNA Stool shows best performance in qPCR
The NucleoSpin RNA Stool samples as well as the Q samples were eluted with 100 μL, the Z eluate was adjusted to 100 μL to get comparable RNA concentrations in the eluate. 1 μL of eluate was used for qRT-PCR performed on a Roche LightCycler using the SensiFAST™ SYBR No-Rox One Step Kit (amplicon size 466 bp). NucleoSpin RNA Stool showed a better performance in the qRT-PCR compared to the two competitor kits.
The gut's diverse microbiome has a huge impact on human health. The use of mRNA sequencing to analyze the transcriptional activity of microorganisms (metatranscriptomics) provides an exciting tool to learn more about their activity.
NucleoSpin RNA Stool kit enables efficient isolation of high quality RNA from any kind of stool sample: