NucleoBond RNA Soil Midi kit for RNA from soil
*taxes and shipping not included
Delivery time approx. 5 working days
Easy handling and superior speed for metagenomic soil analysis, MN Bead Tubes Type A included
Application | Isolation of RNA |
Target | RNA |
CE certified | No, research use only |
Technology | Anion exchange chromatography |
Brand | NucleoBond |
Format | Midi prep |
Handling | Gravity flow |
Lysate clarification | Centrifugation |
Automated use | No |
Sample material | Soil |
Sample amount | < 2 g |
Fragment size | > 100 nt |
Typical yield | 1–10 µg |
Theoretical binding capacity | 600 µg |
Typical purity A260/A280 | 1.7–2.1 |
Typical RIN | > 8.5 |
Elution volume | 100 µL |
Preparation time | 60 min/6 preps |
Typical downstream application | Array technology, Northern blotting, Primer extension, Real−time RT−PCR, RNase protection assays, RNA sequencing |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 27 Month(s) |
Scope of delivery | NucleoBond RNA Columns, NucleoSpin Bead Tubes Type A, NucleoSpin Finisher Columns, buffers |
Gross weight (incl. packaging) | 2400.1 g / 5.29 lbs |
Packaging dimensions | 302 x 220 x 234 mm / 11.89 x 8.66 x 9.21 Inch |
Hazardous material | Yes |
- Download Instruction NucleoBond RNA Soil
- Download Protocol recommendations for viral nucleic acid isolation from water samples
- Download Protocol recommendation sample concentration for viral nucleic acids from water
- Download Flyer NucleoBond RNA Soil
- Download Flyer NucleoBond RNA Soil (ES)
- Download Leaflet
RNA purification from soil samples - NucleoBond RNA Soil
Our NucleoBond RNA Soil and NucleoBond RNA Soil Mini kits enable efficient isolation of high quality RNA and DNA (requires DNA Set for NucleoBond RNA Soil) from any kind of soil or sediment sample. Most soils contain relatively low amounts of RNA, compared to DNA. Therefore we designed our new kit with our proven NucleoBond technology to enable high sample input and pure nucleic acids. With our NucleoSpin Bead Tubes Type A, the optimized Lysis Buffer, and Phenol:Chloroform:Isoamylalcohol an efficient sample disruption is ensured. The optional Buffer OPT enables efficient RNA and DNA purification from mineralic and organic soil samples.
NucleoBond RNA Soil procedure
Application data
High RNA yields with NucleoBond RNA Soil
Different soil samples (clay, shore soil, forest soil) were purified in duplicates according to the standard procedure. For comparison, the samples were applied to a competitor kit from Q. RNA was eluted in 100 μL, and determined photometrically. NucleoBond RNA Soil convinced due to the high RNA yield.
Amplifiable RNA for perfect results with NucleoBond RNA Soil kit.
Duplicates of different soil samples (clay, shore soil, forest soil) were purified in duplicates according to the standard procedure from MN, and Q. 4 μL of eluate was applied to the RT-PCR (amplicon: 466 bp). All MN samples showed lower CP values compared with Q samples, indicating higher RNA yield.