NucleoBond Xtra Midi Plus EF, Midi kit for endotoxin-free plasmid DNA

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740422.10 031010413001
Package unit 10 Preps
Quantity:
The all-in-one solution for endotoxin-free plasmid purification: The NucleoBond Xtra Midi Plus... more

The all-in-one solution for endotoxin-free plasmid purification: The NucleoBond Xtra Midi Plus EF kit also includes the NucleoBond Finalizers. These syringe filters enable fast and straightforward desalting after plasmid DNA has been eluted without the need for a centrifuge.

Application Endotoxin-free plasmid DNA isolation
Selling unit 10 Prep(s), 50 Prep(s)
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Midi prep
Handling Gravity flow
Lysate clarification Column filter
Automated use No
Sample material Bacteria, E. coli
Sample amount < 200 mL (high copy), < 400 mL (low copy)
Vector size 2−50 kbp
Typical yield 500 µg
Theoretical binding capacity 1000 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level ≤ 0.05 EU/µg DNA
Isopropanol precipitation NucleoBond Finalizer
Preparation time 45 min/prep
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15–25 °C / 59–77 °F
Shelf life (from production) 39 Month(s)
Hazardous material Yes