NucleoSpin Plasmid Transfection-grade, Mini kit for ultrapure plasmid DNA

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740490.50 031010411001
Package unit 50 Preps
Quantity:
NucleoSpin Plasmid Transfection-grade is a spin column based kit for manual use. Our advanced... more

NucleoSpin Plasmid Transfection-grade is a spin column based kit for manual use. Our advanced silica membrane based plasmid isolation kit is able to achieve endotoxin levels below ≤ 50 EU/µg plasmid DNA, which makes it viable for transfection of non-sensitive cells. Plasmid DNA is eluted in a slight alkaline buffer (5 mM Tris/HCl, pH 8.5), thus being ready for subsequent applications.

Application Transfection-grade plasmid DNA isolation
Selling unit 10 Prep(s), 50 Prep(s), 250 Prep(s)
Target Plasmid DNA
CE certified No, research use only
Technology Silica membrane technology and endotoxin reduction technology
Brand NucleoSpin
Format Mini prep
Handling Centrifugation, vacuum
Lysate clarification Centrifugation
Automated use No
Sample material Bacteria, E. coli
Sample amount 1−5 mL
Vector size < 25 kbp
Typical yield 15−30 µg
Theoretical binding capacity 35 µg
Typical purity A260/A280 1.8−1.85
Endotoxin level ≤ 50 EU/µg DNA
Elution volume 30−50 µL
Preparation time 20 min/6 preps
Typical downstream application Transfection of sensitive cells and cell injections
Storage temperature 15–25 °C / 59–77 °F
Shelf life (from production) 27 Month(s)
Hazardous material Yes
Resuspension Buffer A1 Resuspension Buffer A1
Content 1000 mL
REF 740911.1
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Lysis Buffer A2 (without LyseControl) Lysis Buffer A2 (without LyseControl)
Content 1000 mL
REF 740912.1
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Neutralization Buffer A3 Neutralization Buffer A3
Content 1000 mL
REF 740913.1
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Wash Buffer AW Wash Buffer AW
Content 1000 mL
REF 740916.1
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Elution Buffer AE Elution Buffer AE
Content 1000 mL
REF 740917.1
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Wash Buffer AQ Concentrate Wash Buffer AQ Concentrate
Content 25 mL
REF 740995
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