EC HPLC column (analytical), NUCLEOSIL 100-5 C18 MPN, 5 µm, 250x4 mm
*taxes and shipping not included
Delivery time approx. 5 working days
Special reversed C18 (ODS) phase with monomerically bonded alkyl chains, brush type structure. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe. Predominantly hydrophobic forces with a small portion of hydrophilic interactions. The dynamic protein binding capacity per g packing - 6 mg BSA, 110 mg cytochrome C. Maximum separation efficiency can be achieved when the injected protein mass does not exceed 1–2 % of the maximum protein loading capacity.
| Method | HPLC, analytical |
| Brand | NUCLEOSIL |
| Base material | NUCLEOSIL - the standard silica |
| Phase | NUCLEOSIL 100-5 C18 MPN |
| USP listing | L1 |
| Surface chemistry | Special octadecyl (C18, ODS) modification |
| Mode | Special application or diverse |
| Type | EC HPLC column (analytical) |
| Hardware | Stainless steel |
| Column length | 250 mm |
| Column inner diameter | 4 mm |
| Particle type | Fully porous particles (FPP) |
| Particle shape | Spherical |
| Particle size | 5 µm |
| Eluent in HPLC column | Methanol |
| Recommended application(s) | Biological macromolecules, HPLC |
| Endcapped | No |
| Carbon content | Not available or proprietary |
| pH stability | 1.0–9.0 |
| Pore size | 100 Å |
| Specific surface according to BET | 350 m²/g |
| Temperature stability (max) | 60 °C |
| Pressure stability (max) | 250 bar / 3625 psi |
| Storage temperature | 15–25 °C / 59–77 °F |
| Scope of delivery | 1x analytical HPLC column, instruction leaflet, certificate of analysis |
| Gross weight (incl. packaging) | 238.77 g / 0.52 lbs |
| Packaging dimensions | 405 x 117 x 54 mm / 15.94 x 4.61 x 2.13 Inch |
| Hazardous material | No |