VP HPLC column (preparative), NUCLEOGEN 4000-7 DEAE, 7 µm, 125x10 mm

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736602.100 0240503030
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Silica-based HPLC phase for anion exchange chromatography of nucleic acids. For the separation... more

Silica-based HPLC phase for anion exchange chromatography of nucleic acids. For the separation of plasmids, DNA restriction fragments, ribosomal RNA, messenger RNA and viral RNA, i.e., very high molecular weight nucleic acids (e.g., 1–50 megadaltons). Capacity 120 A260 for a 125 x 6 mm ID column, 350 A260 for a 125 x 10 mm ID column.

Method HPLC, preparative
Brand NUCLEOGEN
Base material Silica gel
Phase NUCLEOGEN 4000-7 DEAE
USP listing Not available or proprietary
Surface chemistry Silica gel based anion exchanger with hydrophilic coating
Mode Ion exchange (IEX), Special application or diverse
Type VP HPLC column (preparative)
Hardware Stainless steel
Column length 125 mm
Column inner diameter 10 mm
Particle type Fully porous particles (FPP)
Particle shape Spherical
Particle size 7 µm
Eluent in HPLC column Methanol
Recommended application(s) DNA restriction fragments, HPLC, Messenger RNA, Plasmids, Ribosomal RNA, Very high molecular weight nucleic acids (e.g., 1–50 megadaltons), Viral RNA
Endcapped No
Carbon content Not available or proprietary
pH stability 2.5–8.0
Pore size 4000 Å
Specific surface according to BET Not available or proprietary
Temperature stability (max) 70 °C
Pressure stability (max) 250 bar / 3625 psi
Storage temperature 15–25 °C / 59–77 °F
Scope of delivery 1x semi-preparative HPLC column, instruction leaflet, certificate of analysis
Gross weight (incl. packaging) 415.8 g / 0.91 lbs
Packaging dimensions 235 x 117 x 54 mm / 9.25 x 4.61 x 2.13 Inch
Hazardous material No