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NucleoBond AX 500, Maxi columns kit for transfection-grade plasmid DNA
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Item number: 740531.50 031010410001
Package unit 50 Piece(s) 1st generation anion exchanger for purification of plasmid DNA more
1st generation anion exchanger for purification of plasmid DNA
Application | High and low copy plasmid DNA purification |
Selling unit | 10 Piece(s), 50 Piece(s) |
Target | Plasmid DNA |
CE certified | No, research use only |
Technology | Anion exchange chromatography |
Brand | NucleoBond |
Format | Maxi prep |
Handling | Gravity flow |
Lysate clarification | Folded filter |
Automated use | No |
Sample material | Bacteria, E. coli |
Sample amount | 30−150 mL (high copy), 100−500 mL (low copy) |
Vector size | < 300 kbp |
Typical yield | 400−500 µg |
Theoretical binding capacity | 500 µg |
Typical purity A260/A280 | 1.8−1.95 |
Endotoxin level | 1−10 EU/µg DNA |
Isopropanol precipitation | Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi |
Preparation time | 80−90 min/4 preps |
Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 72 Month(s) |
Hazardous material | No |
NucleoBond Xtra Maxi Columns for transfection-grade plasmid DNA
Content 10 Piece(s)
REF 740414.10S
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