NucleoBond PC Prep scale purification of endotoxin-free plasmid DNA

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Item number: 740594 031010410001

Package unit 1 Preps

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Preparative scale isolation of endotoxin-free plasmid DNA

Application	High and low copy plasmid DNA purification
Target	Plasmid DNA
CE certified	No, research use only
Technology	Anion exchange chromatography
Brand	NucleoBond
Format	Preparative scale
Handling	HPLC, FPLC
Lysate clarification	Folded filter, sieving fabric
Automated use	No
Sample material	Bacteria, E. coli
Sample amount	5−20 L
Vector size	< 300 kbp
Typical yield	80−100 mg
Theoretical binding capacity	100 mg
Typical purity A260/A280	1.8−1.95
Endotoxin level	< 0.1 EU/µg DNA
Isopropanol precipitation	Centrifugation
Preparation time	20 h/prep
Typical downstream application	Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature	15–25 °C / 59–77 °F
Shelf life (from production)	39 Month(s)
Scope of delivery	NucleoBond AX Prep 100 Column, NucleoBond AX 100 Columns, NucleoBond Folded Filters (Type 1 and 2), buffers, RNase A
Gross weight (incl. packaging)	10596.35 g / 23.36 lbs
Packaging dimensions	365 x 290 x 280 mm / 14.37 x 11.42 x 11.02 Inch
Hazardous material	Yes