NucleoMag Desalting Beads
*taxes and shipping not included
Delivery time approx. 5 working days
Convenient and scalable desalting of anion exchange eluates using magnetic beads.
Application | Concentration and desalting of plasmid eluates |
Technology | Magnetic bead technology |
Brand | NucleoMag |
Sample material | Bacteria, E. coli |
Target | Plasmid DNA |
Format | Magnetic beads |
CE certified | No, research use only |
Automated use | Yes |
Preparation time | 25 min/prep |
Typical downstream application | Sequencing |
Sample amount | 5 mL NucleoBond Xtra Midi eluate, scalable from 1 - 100 mL |
Typical recovery | ≥90 % |
Elution volume | 200−2000 µL (depending on plasmid DNA concentration and amount) |
Handling | Manual or automated |
Storage temperature | 15–25 °C / 59–77 °F |
Gross weight (incl. packaging) | 112.3 g / 0.25 lbs |
Packaging dimensions | 53 x 53 x 104 mm / 2.09 x 2.09 x 4.09 Inch |
Hazardous material | No |
Plasmid DNA - Desalting and Concentraction
Following the elution of pure plasmid DNA from the NucleoBond columns, MN provides a range of options for desalting and concentrating the plasmid. In addition to the standard isopropanol precipitation followed by centrifugation we offer the NucleoBond Finalizers for manaul desalting with a syringe as well as centrifuge-compartible NucleoSPin Finisher and vacuum compartible NucleoSnap Finisher for a fast, simple and easy prep.
Isopropanol precipitation (traditional method, duration 1 h)
NucleoBond Finalizer and Finazlizer Large (ideal for small number of samples, duration 5 min/sample)
NucleoMag Desalting Beads (can be automated on the Andrew+ in combination with NucleoBond Xtra Midi kits)
NucleoSpin Finisher (for parallel processing of samples, with appropriate centrifuge, duration < 10 min/12 samples)
NucleoSnap Finisher Midi and Maxi (for parallel processing of samples, vacuum manifold, duration < 10 min/12 samples)
NucleoBond Finalizer
NucleoSpin and NucleoSnap Finisher
NucleoSpin Finisher are designed for quick concentration and desalting of plasmid eluates obtained by anion-exchange chromatography. Finisher replace tedious and time-consuming isopropanol precipitation. Furthermore, problems like lost DNA pellets or incomplete solubilization of hardly visible precipitates can easily be circumvented.
NucleoMag Desalting Beads
NucleoMag Desalting beads are designed for quick concentration and desalination of plasmid DNA eluates that are obtained be anion exchange chromatographic plasmid purification kits. Plasmid DNA is bound reversibly to NucleoMag Desalting beads followed by ethanolic wash steps. Finally pure plasmid DNA is eluted using a low salt buffer. NucleoMag Desalting beads can be automated on e.g the Andrew+ platform.
The NucleoBond Finalizers are designed to concentrate and desalinate plasmid DNA eluates collected using NucleoBond columns after anion-exchange chromatographic DNA purification. The sample is syringe-loaded onto a NucleoBond Finalizer membrane to avoid tedious centrifugation steps.