NucleoSpin 96 DNA Stool Core Kit, 96-well kit for DNA from stool
*taxes and shipping not included
Isolation of total DNA from stool samples in 96‑well plate format
Application | Isolation of DNA |
Selling unit | 384 Prep(s), 2304 Prep(s) |
Target | DNA |
CE certified | No, research use only |
Technology | Silica membrane technology |
Brand | NucleoSpin |
Format | 96-well plate |
Handling | Vacuum, centrifugation |
Lysate clarification | Vacuum, NucleoSpin Stool Filter Plate |
Automated use | Yes |
Automation support | Compatible with most open robotic platforms. Please visit www.mn-net.com/automation or consult our technical support for further information. |
Sample material | Stool |
Fragment size | 50 bp–approx. 50 kbp |
Typical yield | 3–15 μg (varies by sample and disruption device) |
Theoretical binding capacity | 50 µg |
Typical purity A260/A280 | 1.6–1.8 |
Elution volume | 100−200 µL |
Preparation time | 90 min/48 samples (automated use) |
Typical downstream application | Metagenomics, NGS, qPCR |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 24 Month(s) |
Hazardous material | Yes |
MACHEREY‑NAGEL has developed a 96-well kit, NucleoSpin 96 DNA Stool, for the reliable, automation-friendly isolation of high quality DNA from human and animal stool samples. The kit combines a robust filtration step, significantly reducing the chance of column clogging, with an efficient DNA extraction procedure based on silica membrane technology.
Reliable performance and excellent yields
![](https://www.mn-net.com/media/image/f0/52/bd/NS-DNA-Stool_96_eppendorfrhSbHo7dlxvGw.png)
![](https://www.mn-net.com/media/image/32/b1/9b/NS-DNA-Stool_96_eppendorf_1.png)
Comparable performance both manually and on epMotion 5075v
Six individual human stool samples were processed manually and on epMotion 5075v in quadruplicates. Yield (blue/grey bars) and purity (A260/280 nm, orange dots) were measured for all of the preparations. The manual and automated extractions delivered comparable yields with high purity (A260/280 > 1.7 in all samples)
High quality DNA from rat stool samples
Eight individual rat stool samples were processed on epMotion 5075v. Yield (blue bars), purity (A260/280 nm, orange dots) and analysis of DNA on the gel indicate the reliably high quality of the extracted DNA.
![](https://www.mn-net.com/media/image/f8/e0/45/NS-DNA-Stool_96_eppendorf_2.png)
![](https://www.mn-net.com/media/image/06/56/0f/NS-DNA-Stool_96_eppendorf_3.png)
No downstream inhibition
DNA was purified from a human stool sample on epMotion 5075v using NucleoSpin 96 DNA Stool. A series of 1:4 dilutions, ranging from the undiluted eluate to a 4096fold dilution was analyzed by qPCR using an E.coli specific target and compared against a theoretical standard in a logarithmic plot. The slope of the regression curve (-3.4106) shows an excellent qPCR-performance without PCR inhibition.
Consistently excellent PCR performance
Six individual human stool samples were processed manually and on epMotion 5075v in quadruplicates. From each eluate, 2,5 µL were used in a 25 µL endpoint PCR reaction, amplifying a 1,5 kb fragment of the bacterial 16SrRNA gene. Three µL from each PCR reaction were analyzed on a 1 % agarose/TAE gel. In each case, the samples were successfully amplified, providing a good indication for the robust performance of the DNA in down‑ stream applications.
NucleoSpin 96 DNA Stool
![](https://www.mn-net.com/media/image/ea/f1/41/Icon_96-well_platezYGJdytta2yca.jpg)