TLC glass plates, silica gel layer, DURASIL UV254, 20x20 cm

TLC glass plates, silica gel layer, DURASIL UV254, 20x20 cm
$245.00 * $0.00 * (0% Saved)

*taxes and shipping not included

Delivery time approx. 5 working days

812008 02601033101010
Package unit 25 Piece(s)
Quantity:
Price overview
Quantity Price/unit
To 9 $245.00
From 10 $216.00
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TLC glass plates in different plate sizes for many different applications. Coated with... more

TLC glass plates in different plate sizes for many different applications. Coated with unmodified silica gel (SiOH). Hard layer that shows other results than SIL G, ADAMANT or SIL HD layers. It is often used for TCM applications.

Method Thin layer chromatography (TLC)
Brand DURASIL
Phase DURASIL UV254
Base material Glass plates coated with silica gel
Surface chemistry Unmodified silica gel (SiOH)
Mode Normal phase (NP)
Plate size 20x20 cm
Layer thickness 0.25 mm
Layer hardness Hard
UV indicator Yes
Support material / backing Glass
Particle size 5–17 µm
Particle type Fully porous particles (FPP)
Particle shape Irregular
Pore size 60 Å
Pore volume 0.75 mL/g
Binder Yes
pH stability 2.0–8.0
Recommended application(s) Anilines, Bile acids, Normal phase (NP), Skin lipids, Steroids
Temperature stability High
Storage temperature 15–25 °C / 59–77 °F
Scope of delivery 25x TLC glass plates (20x20 cm)
Gross weight (incl. packaging) 3537 g / 7.8 lbs
Packaging dimensions 237 x 235 x 80 mm / 9.33 x 9.25 x 3.15 Inch
Hazardous material No
Learn more

MACHEREY‑NAGEL – TLC for more than 5 decades

We offer ready-to-use layers for TLC and HPTLC

  • Comprehensive range of plate sizes, surface chemistries and backings
  • Pre-coated plates ready for immediate use
  • Homogeneous, smooth and well adhering layers
  • Available with UV indicator or non-impregnated
  • Consistent high quality from batch-to-batch and from plate-to-plate

Standard analytical TLC plates and sheets

Thin layer chromatography can be used for both qualitative and quantitative analysis. Standard analytical TLC plates typically have adsorbent layers that are nominally between 0.20–0.25 mm in thickness. Particle size is about 5–17 µm.

Preparative TLC plates

Preparative TLC is used for purification and isolation of analytes from impurities. Preparative TLC layers (≥ 0.5 mm) are only on glass plates available. For 2 mm preparative layers a slightly coarser material is used.

TLC / HPTLC plates and sheets

Most commonly used silica gel layers

SIL G

  • Standard layer (soft)
  • Thicker layers for preparative TLC (PLC) on glass plates
  • Available on glass plates, polyester and aluminum sheets

ADAMANT

  • Outstanding hardness and abrasion resistance
  • Excellent separation efficiency
  • Low-noise background of the layer
  • Available on glass plates

SIL HD

  • Hard layer with good abrasion resistance
  • High luminosity
  • Brilliant staining properties (e.g., with potassium permanganate - KMnO₄)
  • Excellent separation efficiency
  • Good wettability for precise colorization results – even with 100 % aqueous detection reagents
  • Low-noise background of the layer
  • Available on glass plates
Characteristics of backing materials
Physical properties
Materialglasspolyesteraluminum
Thickness (approx.)1.3 mm0.2 mm0.15 mm
Weight, packaging and storage requirementshighlowlow
Torsional strengthideallowrelatively high
Temperature stabilityhighmax. 185 °Chigh
Susceptible to breakageyesnono
Can be cut with scissorsnoyesyes
Chemical resistance of backing material
Against solventshighhighhigh
Against mineral acids and conc. ammoniahighhighlow
Stability of binder system of NP plates in water
Suitability for aqueous detection reagentsdepends on
phases (layer)
goodALUGRAM: low to moderate
ALUGRAM Xtra: moderate to high
Good to know - HPTLC

MN defines HPTLC products as follows

At least one of the following criteria must be fulfilled

  • Layers with particle size 2–10 µm
  • Layers with prefix "Nano"
  • All modified layers

Narrow fractionation of the silica particles allows

  • Sharper separations
  • Shorter developing times
  • Shorter migration distances
  • Smaller samples needed
  • Increased detection sensitivity
    (compared to TLC)
Indicators and binder systems
  • Indicators: manganese activated zinc silicate with green fluorescence for short-wave UV (254 nm); special inorganic fluorescent pigment with blue fluorescence for long-wave UV (366 nm)
  • Binders: highly polymeric products, which are stable in almost all organic solvents and resistant towards aggressive visualization reagents. The binders are also contain gypsum (calcium sulfate hemi- or dihydrate). The binder composition is proprietary.

TLC / HPTLC backings

Glass

Glass plates are robust, heat proof and chemically resistant to all common mobile phases and visualization reagents.

  • Thickness (approx.): 1.3 mm
  • Weight and storage requirements: high
  • Torsional strength: ideal
  • Temperature stability: high
  • Susceptible to breakage: yes
  • Can be cut with scissors: no
  • Chemical resistance against solvents: high
  • Chemical resistance against mineral acids and conc. ammonia: high
  • Suitability for aqueous detection reagents: depends on phases (layer)

Polyester – POLYGRAM sheets 

Polyester sheets are easy to handle, lightweight and flexible. Can be cut with scissors. Developed POLYGRAM sheets can also be stored for documentation in laboratory notebooks.

  • Thickness (approx.): 0.2 mm
  • Weight and storage requirements: low
  • Torsional strength: low
  • Temperature stability: max. 185 °C
  • Susceptible to breakage: no
  • Can be cut with scissors: yes
  • Chemical resistance against solvents: high
  • Chemical resistance against mineral acids and conc. ammonia: high
  • Suitability for aqueous detection reagents: good

Aluminum – ALUGRAM (Xtra) sheets

Aluminum sheets are easy to handle, lightweight and flexible. High performance silica on ALUGRAM Xtra sheets provides outstanding wettability for precise colorization results, even with 100 % aqueous detection reagents. Moreover ALUGRAM Xtra sheets are easy to cut with scissors. No flaking of silica occurs!

  • Thickness (approx.): 0.15 mm
  • Weight and storage requirements: low
  • Torsional strength: relatively high
  • Temperature stability: high
  • Susceptible to breakage: no
  • Can be cut with scissors: yes
  • Chemical resistance against solvents: high
  • Chemical resistance against mineral acids and conc. ammonia: low
  • Suitability for aqueous detection reagents: ALUGRAM: low to moderate, ALUGRAM Xtra: moderate to high

Short introduction video (EN) about TLC and HPTLC products from MACHEREY-NAGEL

How to cut our TLC sheets

The video explains the correct position of the scissors for a clean cut of a TLC/HPTLC sheet.

Please note that the scissors must be held slightly to the right when cutting. If the scissors are tilted to the left or held straight, the coating may flake off.

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