VP HPLC column (preparative), NUCLEODUR PFP, 5 µm, 50x10 mm

VP HPLC column (preparative), NUCLEODUR PFP, 5 µm, 50x10 mm
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762210.100 02403028003505
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This hydrophobic phase with pentafluorophenyl-propyl modification is based on high purity... more

This hydrophobic phase with pentafluorophenyl-propyl modification is based on high purity NUCLEODUR silica gel and gives an alternative selectivity in comparison to classical C18 modifications. Separation mechanism based on four retention mechanisms - polar interactions (H bonds), dipole-dipole interactions, π-π interactions and hydrophobic interactions. Suitable for LC/MS due to low bleeding characteristics.

Method HPLC, preparative
Brand NUCLEODUR
Base material NUCLEODUR - high purity silica
Phase NUCLEODUR PFP
USP listing L43
Surface chemistry Pentafluorophenylpropyl (PFP) modification
Mode Reversed phase (RP)
Type VP HPLC column (preparative)
Hardware Stainless steel
Column length 50 mm
Column inner diameter 10 mm
Particle type Fully porous particles (FPP)
Particle shape Spherical
Particle size 5 µm
Eluent in HPLC column Acetonitrile – water
Recommended application(s) For examples please see analytical NUCLEODUR PolarTec, RP applications (preparative)
Endcapped Yes
Carbon content 8.0 %
pH stability 1.0–9.0
Pore size 110 Å
Specific surface according to BET 340 m²/g
Temperature stability (max) 60 °C
Pressure stability (max) 400 bar / 5800 psi
Column volume 3.9 mL
Recommended flow rate 3–9 mL/min
Storage temperature 15–25 °C / 59–77 °F
Scope of delivery 1x preparative HPLC column, instruction leaflet, certificate of analysis
Hazardous material No