NucleoSpin VET for viral and bacterial DNA and viral RNA from veterinary samples
*taxes and shipping not included
Mini spin kit for the isolation and purification of animal pathogen nucleic acids from veterinary materials
| Application | Isolation and purification of animal pathogen nucleic acids from veterinary materials |
| Selling unit | 10 Prep(s), 50 Prep(s), 250 Prep(s) |
| Target | Bacterial DNA, Viral RNA and DNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology |
| Brand | NucleoSpin |
| Format | Mini prep |
| Handling | Centrifugation |
| Lysate clarification | Centrifugation, NucleoSpin Filter |
| Automated use | No |
| Sample material | Animal tissue, Blood, Cell-free biological fluid, Milk, Plasma, Serum, Stool, Swabs |
| Sample amount | < 200 µL serum, plasma, cell-free biological fluids, milk, 100 μL blood, 5–10 mg tissue, one dry or wet swab, or approx. 100 mg feces |
| Fragment size | 100 bp−approx. 50 kbp |
| Theoretical binding capacity | 25 µg |
| Preparation time | 20-40 min |
| Typical downstream application | enzymatic reactions, NGS, qPCR, qRT−PCR |
| Shelf life (from production) | 27 Month(s) |
| Hazardous material | Yes |
NucleoSpin VET - silica membrane-based sample extraction
The NucleoSpin VET kit is a single spin silica membrane-based kit for the isolation of viral RNA/DNA and bacterial DNA from diverse veterinary sample specimens such as serum, plasma, cell-free biological fluids, animal whole blood, tissue, swabs, milk and feces. The versatile range of applications brings maximum flexibility to your daily laboratory routine and allows the processing of a wide variety of sample materials using the same standard protocol after sample pretreatment.
- Inhibitor free viral RNA/DNA and bacterial DNA extraction
- Highest flexibility for various veterinary sample types
- Same standard protocol for all specimen matrices after sample pretreatment
- Fast track 20 min protocol for rapid routine testing of low complexity sample matrices
NucleoMag VET - magnetic bead-based sample extraction
The NucleoMag VET kit is a magnetic bead-based kit for the manual or automated extraction of viral RNA/DNA and bacterial DNA from veterinary sample specimens such as serum, plasma, cell-free biological fluids, animal whole blood, tissue, swabs, milk and feces. After sample treatment, samples can be processed with the standard purification protocol.
- Inhibitor free viral RNA/DNA and bacterial DNA extraction
- Highest flexibility for various veterinary sample types
- Same standard protocol for all specimen matrices after sample pretreatment
- Easy to automate on magnetic rod or liquid handler systems - Contact us for assistance
Automated sample extraction made easy!
Are you planning to automate your sample preparation? The automation specialists at MACHEREY-NAGEL can support you with implementing the NucleoMag VET kit on your robotic platform. Contact us for support or for free and ready-to-go scripts, e.g. for KingFisher instruments or other magnetic rod and liquid handler platforms:
E-Mail: bio-tech@mn-net.com I Tel.: +49 24 21 969 270
Nucleic acid isolation for veterinary diagnostics
Veterinarians use a variety of tools to diagnose diseases, monitor disease progression or response to therapy, and screen apparently healthy animals for the presence of underlying disease. Many of these methods rely on molecular biology detection techniques such as qPCR or sequencing.
MACHEREY-NAGEL has therefore developed specific products for the extraction of pathogenic nucleic acids such as viral RNA/DNA or bacterial DNA. These DNA/RNA extraction kits are suitable for virtually all veterinary samples, provide high quality nucleic acids with excellent purity, and are available in manual or automatable formats:
Reliable viral DNA and RNA recovery with NucleoSpin VET kit from bovine blood
The NucleoSpin VET kit was used for viral DNA and RNA isolation from bovine EDTA blood with diverse virus load. The thawed bovine blood was spiked with LSDV (lumpy skin disease virus; DNA virus), BoCoV and FMDV (bovine corona virus, foot-and-mouth-disease virus; RNA viruses) at various dilution levels. The samples were purified using the NucleoSpin VET kit following the standard protocol procedure and eluates were subsequently analyzed via RT-qPCR using AgPath-IDTM One-Step RT-PCR reagents (ThermoFisher Scientific). The NucleoSpin VET kit shows consistent and reliable viral RNA and DNA recovery from high to low viral titer samples (FMDV titer in 1:10000 dilution was below detection limit).
Source: Product Broschure NucleoSpin VET; data kindly provided by Hanna Keck and Michael Eschbaumer, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health of Germany.
RT-qPCR detection of foot-and-mouth disease virus in bovine milk and skin lesions
To compare manual and automated extraction, the NucleoSpin VET kit and the NucleoMag VET kit were used to isolate viral RNA from known FMDV-positive samples (foot-and-mouth-disease virus). The samples were purified using the NucleoSpin VET kit following the standard protocol procedure and in parallel with the magnetic bead based NucleoMag® VET kit (100 µL sample input). Eluates were subsequently analyzed via RT-qPCR using AgPath-IDTM OneStep RT-PCR reagents (ThermoFisher Scientific). The results show that both kits performed comparably well, even with difficult samples such as very fatty milk or skin lesions.
Source: Product Broschure NucleoSpin VET; data kindly provided by Hanna Keck and Michael Eschbaumer, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health of Germany.
Reliable detection of BTV, CSFV, PCV 2, and SBV after DNA or RNA isolation using the NucleoMag VET kit
The NucleoMag VET kit was used to extract viral nucleic acids from bovine blood (A), porcine blood (B), porcine tissue (c) or bovine serum (d). Subsequently, qPCR-analysis was performed to determine the viral titer load in the sample materials: (A) BTV (Bluetongue virus); (B) CSFV (Classical Swine Fever virus) (C) PCV 2 (Porcine Circovirus Type 2); (D) SBV (Schmallenberg virus). Extraction performance was compared to other commercial nucleic acid extractions kits available for isolation of viral RNA and/or DNA. The qRT-PCR data show that DNA or RNA concentration varies on the extraction kit used, which results in a dissimilar detection of BTV, CSFV, PCV 2, and SBV. For the detection of the various viruses in the disparate sample materials, it is shown that DNA or RNA concentration was always high using the NucleoMag VET compared to the other kits. The obtained Ct values for the viral nucleic acids and sample materials show that the NucleoMag VET kit is a very consistent and reliable extraction kit, obtaining low Ct values for a reliable virus detection independent from virus type or sample material.
Source: Application note NucleoMag VET on KingFisher; data was kindly provided by Dr. Hoffmann, Friedrich-Loeffler-Institut, Germany.
Further application data for the NucleoMag VET kit can be found via the scientific references in the literature list or in the application notes below!
Cited in over 100 peer-reviewed publications!
The NucleoMag VET kit has been cited in more than 100 peer-reviewed publications. The magnetic bead-based kit has been used in research and molecular diagnostics for a variety of pathogens relevant to veterinary medicine. Click here to see the full reference list:
- African/classical swine fever virus
- Lumpy skin disease virus
- Petits ruminants virus
- Influenza A virus
- Foot-and-mouth disease virus
- Schmallenberg virus
Example application notes:
NucleoMag VET kit for viral/bacterial DNA and viral RNA from veterinary samples 
NucleoProtect VET Reagent, DNA / RNA stabilization and sample inactivation 
NucleoProtect VET Blood Tube, DNA / RNA stabilization and sample inactivation 
NucleoProtect VET Swab Tube, DNA / RNA stabilization and sample inactivation 
