NucleoType HotStart PCR Master Mix
*taxes and shipping not included
Delivery time approx. 5 working days
The NucleoTypeR HotStart PCR Master Mix is a high-performance PCR solution combining Polymerase, dNTPs, buffer, enhancer, stabilizer, and colorant. It enhances PCR yield, specificity, and speed through advanced buffer chemistry and polymerase technology. The hot-start enzyme ensures exceptional sensitivity, particularly for complex templates like mammalian or bacterial genomic DNA. With its robust enzyme and buffer system, it's ideal for multiplex PCR assays, allowing direct gel loading due to the colorant. Recommended for colony PCR reactions or in combination with NucleoType® genotyping products, it's a versatile tool for molecular biology research.
Application | colony PCR, Direct PCR, genotyping, multiplex PCR |
Brand | NucleoType |
Target | DNA |
Format | PCR |
CE certified | No, research use only |
Scope of delivery | 2.5 mL 2x master mix (2 x 1.25 mL) for up to 500 reactions, Polymerase, dNTPs, buffer, Enhancer, Stabilizer, Colarant |
Storage temperature | 2–8 °C / 36–46 °F |
Gross weight (incl. packaging) | 24.2 g / 0.05 lbs |
Packaging dimensions | 53 x 53 x 104 mm / 2.09 x 2.09 x 4.09 Inch |
Hazardous material | No |
Molecular cloning workflow solutions
- Recombinant DNA technology solutions
- From colony PCR to protein expression analysis
- Trustworthy tools for molecular cloning success
Molecular Cloning
Molecular cloning for plasmid construction describes the process of inserting a
specific DNA fragment into a plasmid vector, typically a bacterial plasmid, to produce
recombinant DNA molecules for various applications in genetic research. Discover
MACHEREY‑NAGEL’s tailored solutions for recombinant DNA technique.
Colony PCR
In the initial phases of molecular cloning, MN presents the NucleoType HotStart PCR Master Mix tailored for colony PCR and genotyping. This pivotal method efficiently screens bacterial colonies, enabling the precise identification of specific DNA fragments. By swiftly pinpointing colonies containing desired genetic material, colony PCR streamlines subsequent selection processes for further analysis.
Colony PCR with direct-to-gel analysis
Gel and PCR Clean up
After colony PCR, agarose gel separation, insert amplification, or other enzymatic reactions, cleaning up DNA fragments is a crucial step in recombinant DNA construction. Our procedures ensure the highest recovery and integrity of DNA samples by efficiently eliminating impurities and contaminants, laying a dependable oundation for subsequent cloning processes.
Gel and PCR Clean-up Kits
NucleoSpin Gel and PCR Clean-up
NucleoSpin Gel and PCR Clean-up XS
NucleoSEQ kit for dye terminator removal
NucleoMag NGS Clean-Up and Size Select
(e. g. Clean-up of enzymatic reactions)
Plasmid Purification
Isolating high-quality plasmid DNA is essential for successful cloning experiments. Our
dedicated plasmid purification protocols enable the extraction of DNA from bacterial
cultures, guaranteeing optimal yields and purity levels essential for various molecular
biology applications, including cloning and sequencing.
Single Plasmid Mini Prep Kits
HTP Plasmid Mini Prep Kits
Plasmid Midi/Maxi Prep Kits
Plasmid Mega Prep Kits
Plasmid Giga Prep Kits
Transfection of sensitive cell lines
In scenarios where experimental integrity is critical, such as transfection of sensitive
cell lines, we offer specialized plasmid purification techniques. By thoroughly removing
endotoxins from plasmid DNA preparations, we reduce potential sources of interference,
ensuring the reliability and reproducibility of downstream experiments.
For transfection of sensitive cells we recommend our transfection-grade or endotoxin-free kits.
Recombinant protein purifciation and characterization
For researchers working with recombinant proteins, purification is a crucial step in the
process. Our suite of purification methods, including affinity chromatography, enables
the isolation of target proteins from complex mixtures, facilitating the acquisition of pure
protein samples suitable for comprehensive characterization and analysis.
Protino products are available in resin or
pre-packed column format.
Protino Ni-NTA Agarose (His-tag proteins)
Protino Glutathione Agarose 4B (GST-tag proteins)
Protino Purification Plates
Protino Columns (14 / 35 mL empty columns)
Transfer membranes and blotting papers. Large selection of various membrane materials.
Click here and read more about our products for protein purification.
You need transfer membranes, blotting papers or materials for surface protection click here for more information.